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Briefly, cells were grown in YPD until logarithmic phase, harvested by centrifugation at 1000 g for 3 min at room temperature, and resuspended in 200 µl of ‘One-step-transformation’ buffer containing 40% polyethylene glycol 3350 (Sigma Aldrich, St

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Annu Rev Cell Dev Biol 30:503–533 [DOI] [PubMed] [Google Scholar] Newman JC, Verdin E (2014) Ketone bodies as signaling metabolites