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It appears to be carried out by the participation of all mature liver cell types.43, 44 The process is associated with signaling cascades involving growth factors, cytokines, matrix remodeling, and several feedbacks of stimulation and inhibition of growth related signals.45, 46 The liver manages to restore any lost mass and adjust its size to that of the organism, while at the same time providing full support for body homeostasis during the entire regenerative process
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In short, organoids were incubated overnight at 4 °C with primary antibodies binding ZO-I (Thermo Fisher Scientific catalog number 61-7300, RRID: AB_2533938), ITGB1 gene product (R and D Systems catalog number MAB1778, RRID: AB_357909) and Ki67 (Millipore catalog number AB9260, RRID: AB_2142366) or ITGA2 -encoded gene (Abcam catalog number ab30487, RRID: AB_775702)